Assembly and Dynamic Interactions of a 1 MDa Membrane Metalloenzyme Machinery Catalyzing a Key Step in Global Aromatic Ring Degradation

Project Summary

During the degradation of the worldwide second most abundant class of organic molecules, aromatic compounds, the dearomatizing benzoyl-CoA reductase (BCR) catalyses a redox reaction below the negative limit of the biological redox scale. Strictly anaerobic bacteria employ a membrane-bound 1 MDa BCR complex to accomplish this reaction; it represents with more than 50 redox cofactors one of the most complex metalloenzyme machineries in nature. The project aims to understand how the assembly and dynamic interactions of individual modules accomplish electron-bifurcation driven electron transfer at an extremely low redox potential.