Dynamic assembly of the Atg1 kinase complex during autophagosome biogenesis

Project Summary

Autophagy is a cellular transport pathway in which cytoplasmic components are targeted for degradation. Defects in autophagy have been associated with several human diseases, including neurodegenerative disorders and cancer. A highly conserved protein kinase, Atg1, plays a unique and essential role in initiating autophagy, yet its regulation and action are not well understood. Atg1 kinase assembles into a dynamic multi-protein complex. It acts as molecular machinery in autophagy by initiating the formation of the autophagic membrane. Here we want to understand how the Atg1 kinase complex is activated and how it regulates downstream events.