Regulation of Biogenesis and Function of the Mitochondrial Protein Import Machinery by Phosphorylation

Project Summary

The mitochondrial protein import machinery mediates import of >1000 different precursor proteins from the cytosol and has been considered long time to be constitutively active. In initial studies we could show that the central preprotein entry gate at the outer membrane, the TOM complex, is regulated by cytosolic protein kinases to adjust the influx of precursor proteins to cellular demands. Here, we aim to study the role of reversible phosphorylation of inner mitochondrial protein import machineries and decipher novel signaling mechanisms involving kinases and phosphatases within the organelle.