Identification and characterization of MOF interaction and substrate repertoire in mammalian cells

Project Summary

Histone acetylation is a prominent epigenetic modification playing diverse roles in several cellular processes. MOF (Males absent On the First) is the major lysine acetyl transferase responsible for deposition of histone H4 lysine 16 (H4K16ac) in flies and mammals. Here we plan to study whether MOF resides in distinct multiprotein assemblies inside and outside the nucleus. We also plan to identify acetylation targets of MOF beyond histones.