Functional and Regulatory Network of the Mitochondrial Presequence Processing Machinery

Project Summary

The mitochondrial proteome is mainly built up by import of cytosolic precursor proteins. Upon import the majority of precursors require proteolytic processing by the presequence processing machinery, that ensures functional mature proteins. Interacting proteins involved in regulation and adaptation of this machinery remain elusive. Here we aim to identify and functionally characterize the interactome of the presequence processing machinery using biochemical and mass spectrometry-based proteomic approaches to decipher its modulation upon changing cellular demands and its link to specific mitochondrial functions.