Partitioning of the Hsp70 Ssb between Ribosomes and Cytosolic Chaperone Machineries

Project Summary

The yeast Hsp70 chaperone Ssb interacts with ribosomes and nascent chains and mediates co-translational protein folding. Ssb is also involved in the post-translational regulation of the central metabolic signaling kinases SNF1 (homolog of human AMPK) and TORC1/Sch9, which control translation in response to environmental changes. Here we wish to uncover how Ssb partitions between its role as a bona fide component of the translational machinery and its role in the regulation of the very same machinery and unravel how this dual function of Ssb allows yeast to adapt to fluctuating environmental conditions.