Dynamics of LRBA machinery in autophagy and vesicle trafficking

Project Summary

LRBA (LPS-responsive beach-like anchor protein) is part of a large cellular protein complex, essential for proper endosomal trafficking in lymphocytes, including the vesicular mobilization of the immune checkpoint CTLA-4 (cytotoxic T-lymphocytes antigen 4) in human regulatory T cells. However, the LRBA multiprotein complex may dynamically change depending on protein metabolism, cell stimulation and cell type. Here our purpose is to identify the different LRBA protein interactors at various cellular conditions using proteomics analysis. In addition, we aim to understand how the LRBA machinery is assembled, and how it regulates different trafficking routes in B-lymphocytes, such as autophagy and antigen internalization.